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Full Text
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urn:etd:azu_etd_2557_sip1_m
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Title
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Dynamic Contrast-Enhanced Magnetic Resonance Imaging and Fluorescence Microscopy of Tumor Microvascular Permeability
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Author
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Dominique Louise Jennings
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Affiliation
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University of Arizona
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Advisor
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Robert J Gillies, Theodore P Trouard
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Date
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2008-3-04 00:00:00
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Degree
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Ph.D.
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Language
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English
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Major
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Biomedical Engineering
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Keywords
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dynamic contrast enhanced-MRI; intravital fluorescence microscopy; microvascular permeability; vascular volume; dorsal midline skinfold window chamber
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Abstract
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Microvascular permeability is a pharmacologic indicator of tumor response to therapy, and it is expected that this biomarker will evolve into a clinical surrogate endpoint and be integrated into protocols for determining patient response to antiangiogenic or antivascular therapies. The goal of this research is to develop a method by which microvascular permeability (Ktrans) and vascular volume (vp) as measured by DCE-MRI were directly compared to the same parameters measured by intravital fluorescence microscopy in an MRI-compatible window chamber model. Dynamic contrast enhanced-MRI (DCE-MRI) is a non-invasive, clinically useful imaging approach that has been used extensively to measure active changes in tumor microvascular hemodynamics. However, uncertainties exist in DCE-MRI as it does not interrogate the contrast reagent (CR) itself, but the effect of the CR on tissue water relaxivity. Thus, direct comparison of DCE-MRI with a more quantitative measure would help better define the derived parameters. The combined imaging system was able to obtain both dynamic contrast-enhanced MRI data high spatio-termporal resolution fluorescence data following injection of fluorescent and gadolinium co-labeled albumin. This approach allowed for the cross-validation of vascular permeability data, in relation tumor growth, angiogenesis and response to therapy in both imaging systems.
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Hash Code
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SHA-1 SUN 0x0904450a57e57b3df79f8177928553c81be3254e
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Attachments
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